Antigen

Antigen Class

TFs and others

Antigen

SIX1

Cell type

Cell type Class

Muscle

Cell type

SMS-CTR

NA

NA

Sample

source_name

Fusion Negative Rhabdomyosarcoma

cell type

Fusion Negative Rhabdomyosarcoma

genotype

SIX1 KD

chip antibody

SIX1 (Atlas, HPA0011893)

cell line

SMS-CTR

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIPseq libraries were assembled using the KAPA HyperPrep ChIP library kit following manufacturer's settings and were sequenced on an Illumina Nextseq500 machine.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

26555615

Reads aligned (%)

84.1

Duplicates removed (%)

11.5

Number of peaks

4502 (qval < 1E-05)

hg19

Number of total reads

26555615

Reads aligned (%)

83.6

Duplicates removed (%)

11.6

Number of peaks

4413 (qval < 1E-05)